Stem Cells and Their Potential for Clinical Application by Nadja M. Bilko (Editor), Boris Fehse (Editor), Wolfram

By Nadja M. Bilko (Editor), Boris Fehse (Editor), Wolfram Ostertag (Editor), Carol Stocking (Editor)

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By Nadja M. Bilko (Editor), Boris Fehse (Editor), Wolfram Ostertag (Editor), Carol Stocking (Editor)

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Read or Download Stem Cells and Their Potential for Clinical Application (NATO Science for Peace and Security Series NATO Science for Peace and Security Series A: Chemistry and Biology) PDF

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The 1st NATO technology discussion board was once held in Biarritz in September 1990. This Taormina convention is the second one in a chain that we want to be an extended one and that i think that it has equalled the luck of its predecessor. In constructing those conferences the NATO technological know-how Committee desired to assemble best specialists to study fields of sturdy current curiosity.

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Stem cells and Development, in press 25. , Ostertag W. and Baum C. (2002) Persisting multilineage transgene expression in the clonal progeny of a hematopoietic stem cell. Leukemia 16, 1655-1663. 26. , and Baum C. (1999) Design of 5' untranslated sequences in retroviral vectors developed for medical use. Journal of Virology 73, 4083-4089. 27. R. -G. (2002) Highly efficient retroviral gene transfer based on centrifugation-mediated vector pre-loading of tissue culture vessels. Molecular Therapy 5, 473-478.

12mg of total RNA/lane were analyzed. 0 kb CSF-1 message. Results for parental Myl-D7 cells, stroma-dependent subclones derived and stroma-independent mutants are shown: 1, #3i-1; 2, #5i-1; 3, #6i-4; 4, #6i-5; 5, #3i-2; 6, #4i-1; 7, #5i-3; 8, #6i-2; 9, #6i-3; 10, #6i-17; 11, #6i-18; 12, #6i-19; 13, #6i-20; 14, #6i-21; 15, #6i-22; 16, #6i-23; 17, #6i26. Those stroma-independent mutants that are secretors are also indicated. Although additional CSF-1 specific splice variants could be detected at low levels in MS-5 and Myl-D7 mutants with high CSF-1 expression, no mutant/cell line specific splice product could be shown.

VON KEUDELL ET AL. References 1. , Jaenisch R. and Breindl M. (1987) Retrovirus integration and chromatin structure: Moloney murine leukemia proviral integration sites map near DNase I-hypersensitive sites. Journal of Virology 61, 336-343. 2. , Karls U. and Harbers K. (1990) Retroviral integration sites in transgenic Mov mice frequently map in the vicinity of transcribed DNA regions. Journal of Virology 64, 3056-3058. 3. , Crise B. M. (2003) Transcription start regions in the human genome are favored targets for MLV integration.

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