By Nadja M. Bilko (Editor), Boris Fehse (Editor), Wolfram Ostertag (Editor), Carol Stocking (Editor)
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The 1st NATO technology discussion board was once held in Biarritz in September 1990. This Taormina convention is the second one in a chain that we want to be an extended one and that i think that it has equalled the luck of its predecessor. In constructing those conferences the NATO technological know-how Committee desired to assemble best specialists to study fields of sturdy current curiosity.
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Additional info for Stem Cells and Their Potential for Clinical Application (NATO Science for Peace and Security Series NATO Science for Peace and Security Series A: Chemistry and Biology)
Stem cells and Development, in press 25. , Ostertag W. and Baum C. (2002) Persisting multilineage transgene expression in the clonal progeny of a hematopoietic stem cell. Leukemia 16, 1655-1663. 26. , and Baum C. (1999) Design of 5' untranslated sequences in retroviral vectors developed for medical use. Journal of Virology 73, 4083-4089. 27. R. -G. (2002) Highly efficient retroviral gene transfer based on centrifugation-mediated vector pre-loading of tissue culture vessels. Molecular Therapy 5, 473-478.
12mg of total RNA/lane were analyzed. 0 kb CSF-1 message. Results for parental Myl-D7 cells, stroma-dependent subclones derived and stroma-independent mutants are shown: 1, #3i-1; 2, #5i-1; 3, #6i-4; 4, #6i-5; 5, #3i-2; 6, #4i-1; 7, #5i-3; 8, #6i-2; 9, #6i-3; 10, #6i-17; 11, #6i-18; 12, #6i-19; 13, #6i-20; 14, #6i-21; 15, #6i-22; 16, #6i-23; 17, #6i26. Those stroma-independent mutants that are secretors are also indicated. Although additional CSF-1 specific splice variants could be detected at low levels in MS-5 and Myl-D7 mutants with high CSF-1 expression, no mutant/cell line specific splice product could be shown.
VON KEUDELL ET AL. References 1. , Jaenisch R. and Breindl M. (1987) Retrovirus integration and chromatin structure: Moloney murine leukemia proviral integration sites map near DNase I-hypersensitive sites. Journal of Virology 61, 336-343. 2. , Karls U. and Harbers K. (1990) Retroviral integration sites in transgenic Mov mice frequently map in the vicinity of transcribed DNA regions. Journal of Virology 64, 3056-3058. 3. , Crise B. M. (2003) Transcription start regions in the human genome are favored targets for MLV integration.