By Mahesh Uttamchandani, Shao Q. Yao
Small molecule microarrays (SMM) have been brought in 1999 and inside of only a brief span of time, have confirmed themselves as a colourful subsequent new release platform for high-throughput screening. Small Molecule Microarrays: tools and Protocols consolidates an important number of examples, which serve to lead researchers towards ways that SMM know-how could be successfully deployed for multiplexed screening, drug discovery and ligand id. the quantity is geared up into 3 sections overlaying microarray fabrication, imaging modes, and discovery ways. The chapters hide quite a number small molecule library kinds, starting from artificial combinatorial libraries to peptide and carbohydrate libraries, with classes and strategies that may be commonly utilized. Written within the hugely winning Methods in Molecular Biology series, layout, chapters contain introductions in their respective issues, lists of the needful fabrics and reagents, step by step and simply reproducible laboratory protocols in addition to important pointers on troubleshooting and recommendation on warding off identified pitfalls.
Authoritative and state-of-the-art, Small Molecule Microarrays: tools and Protocols presents meticulous insights into the winning purposes of SMM, through specialists within the box. construction in this choice of wisdom, the quantity seeks to motivate a destiny new release of microarray practitioners to propel this crucial expertise to even better heights
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Additional resources for Small Molecule Microarrays: Methods and Protocols
28. Streptavidin-Cy3™ from Streptomyces avidinii buffered aqueous solution: Streptavidin-Cy3 (Sigma-Aldrich). 29. Mouse anti-dextran monoclonal (StemCell Technologies). antibody, clone DX1 30. ). 31. ). 32. Mouse anti-blood group Lewis X monoclonal antibody, clone P12 (GeneTex). 33. Mouse anti-sialyl Lewis X antibody, clone KM93 (Millipore). 34. Anti-dextran monoclonal antibody, clone DX1, FITC conjugated (StemCell Technologies). 35. Goat anti-mouse IgG antibody, Cy3 conjugate (Millipore). 36. Goat anti-mouse IgM antibody, μ chain, Cy3-conjugate (Millipore).
4). 02 % (v/v) Tween 20 in 1× PBS buffer. Bovine serum albumin (BSA) buffer: 5 mg/ml BSA in 1× PBS buffer. Stabilizer: Guardian™ peroxidase conjugate stabilizer/diluent (ThermoFisher Scientific). 4). 2. Protein microarray reagents: human IgG (Beijing Dingguo Changsheng Biotechnology, Catalog Number AG-0012), horseradish peroxidase (HRP) labeled goat anti-human IgG (Beijing Dingguo Changsheng Biotechnology, Catalog Number 1H-0021), and HRP substrate solution (ThermoFisher Scientific). 3. Sandwich enzyme-linked immunosorbent assay (ELISA) for cytokine detection reagents: IL-1β human antibody pair (ThermoFisher Scientific, Catalog Number CHC1213), IL-6 human antibody pair (ThermoFisher Scientific, Catalog Number CHC1263), IL-10 human antibody pair (ThermoFisher Scientific, Catalog Number CHC1323).
40. Mini E-centrifuge (Wealtec). 41. Vortex-2 (Scientific Industries). 42. Pap jar (Pap-Fix Plus, Evergreen). 43. Microarray hybrid chamber (CamLab, UK). 44. 16-Well incubation chamber (Sigma-Aldrich). 45. Robotic contact arrayer (AD1500 Arrayer, BioDot). 46. Stealth Micro Spotting pins SMP3 (Arrayit Corporation). 47. ArrayWorx microarray scanner (Applied Precisions). 48. 0). 49. Microsoft Excel software. 1 Preparation of BA-Derivatized Glass Slides 1. Place commercial aldehyde slides (four slides) into a pap jar containing 5 wt% BSA (25 mL) in deionized water.