By Ivor Smith and J. W. T. Seakins (Eds.)
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Solvent front. Length of Solvent Flow. As the solvent composition is continuously changing it follows that R r values determined after short runs (say 10 cm. solvent flow from the origin) will be different from those obtained after long runs (say 20 cm. flow). Furthermore, as a corollary to the above, the distance of the origin from the liquid solvent surface will affect the R^ value. In all, some nine or ten factors can cause R values to vary both in the same and different apparatus. g. the same apparatus, type of solvent, grade of paper, and direction of grain for the same separation.
J . Anal. , 1951, 23, 415. The Unikit in Chromatography and Electrophoresis on Paper and Thin Layers. Ed. , and Feinberg, J . G. , Sewickley, Pa 15143; Longmans Publishers, London. CHAPTER 3 THIN LAYER CHROMATOGRAPHY Ivor Smith and R. S. Ersser ALTHOUGH a number of workers had previously described procedures for thin layer chromatography, TLC, the first practical description of apparatus and technique was t h a t of Stahl. ( 1 ) His contribution was not limited to this, however, as it was equally important to make available suitable materials for preparing the layer and his investigation of the factors concerning the manufacture of such materials was of at least equal importance.
If a two-way chromatogram is being prepared, the dried paper is trimmed with scissors at each end thus removing the serrations and the part of the paper which was previously folded into the trough. A second lot of serrations are cut on the adjacent side and the paper is folded and replaced in the trough as before. A range of pressed-glass tanks for sizes of paper up to 18 X 22 in. F I G . 4. One of the "Panglas" tanks available in a number of sizes. Note the pressed area at the top suitable for troughs and antisyphon rods.