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Additional info for NMR Spectroscopy Data Acquisition
Currently the calculated coherence transfer processes are limited to polarization transfer and cross polarization. 1 Spin system parameters The most important spin system parameters can often be obtained directly from a NMR spectrum by simply measuring the signal positions, the line separation in multiplets and the linewidths. It must be stressed that a molecular parameter measured directly from a NMR spectrum, particularly in second order spectra, is not necessarily the same as the spin system parameter.
And TPPI method to obtain phase sensitive spectra. 8, it was emphasized that frequency discrimination in the f1 dimension was inherent in phase modulated data sets. However the lineshapes was phase twisted because of the absorptive and dispersive contributions to the real part of s(ω1, ω2) which describes the lineshapes in the final spectrum. The Echo/Antiecho procedure relies on the possibility of generating two experiments simply by changing the sign of a gradient pulse. Thus the applied gradients select in two scans different coherence transfer pathways which contribute to the detected signal.
For instance, if a x-pulse with a tilt angle ϕ ≠ n·π/2 is executed the Iinital operator Iz is transferred to the operator -Iy and a residual Iz. Because the operators are commutable the evolution of chemical shift and scalar coupling can be Ω·t·Iz 2π·t·JISIzSz calculated in any order. So the operations and 2π·t·JISIzSz Ω·t·Iz have the same effect on the spin systems IS. In their current form the CARTESIAN product operators cannot be used to understand phase cycling or gradients because the operators do not have any information about either the coherence level or the phase shift a particular coherence has experienced during the previous pulse sequence.