Microarrays: Volume 2: Applications and Data Analysis by Conor W. Sipe, Margaret S. Saha (auth.), Jang B. Rampal

By Conor W. Sipe, Margaret S. Saha (auth.), Jang B. Rampal (eds.)

Microarray expertise, Volumes 1 and a couple of, current info in designing and fabricating arrays and binding reviews with organic analytes whereas offering the reader with a huge description of microarray expertise instruments and their capability functions. the 1st quantity bargains with tools and protocols for the training of microarrays. the second one quantity information purposes and knowledge research, that's very important in reading the big info popping out of microarray experiments.

Volume 2: purposes and knowledge research contains perception into non-mammalian vertebrate structures, procedures and protocols for top of the range glass-based microarrays. purposes in DNA, peptide, antibody and carbohydrate microarraying, oligonucleotide microarrays generated from hydrolysis PCR probe sequences, microarray systems in scientific perform, and screening of cDNA libraries on glass slide microarrays. Authors during this quantity additionally talk about paraflo biochip for nucleic acid and protein research, volumetric mass spectrometry protein arrays, protocols for predicting DNA duplex balance on oligonucleotide arrays, and built-in research of microarray results.

Microarray expertise, Volumes 1 and a pair of, supply abundant details to all degrees of scientists from amateur to these in detail acquainted with array technology.

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By Conor W. Sipe, Margaret S. Saha (auth.), Jang B. Rampal (eds.)

Microarray expertise, Volumes 1 and a couple of, current info in designing and fabricating arrays and binding reviews with organic analytes whereas offering the reader with a huge description of microarray expertise instruments and their capability functions. the 1st quantity bargains with tools and protocols for the training of microarrays. the second one quantity information purposes and knowledge research, that's very important in reading the big info popping out of microarray experiments.

Volume 2: purposes and knowledge research contains perception into non-mammalian vertebrate structures, procedures and protocols for top of the range glass-based microarrays. purposes in DNA, peptide, antibody and carbohydrate microarraying, oligonucleotide microarrays generated from hydrolysis PCR probe sequences, microarray systems in scientific perform, and screening of cDNA libraries on glass slide microarrays. Authors during this quantity additionally talk about paraflo biochip for nucleic acid and protein research, volumetric mass spectrometry protein arrays, protocols for predicting DNA duplex balance on oligonucleotide arrays, and built-in research of microarray results.

Microarray expertise, Volumes 1 and a pair of, supply abundant details to all degrees of scientists from amateur to these in detail acquainted with array technology.

Show description

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Extra resources for Microarrays: Volume 2: Applications and Data Analysis

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2003) Staudinger ligation: a new immo8. bilization strategy for the preparation of small-molecule arrays. Angew. Chem. Int. Ed. 42, 5830–5834. 9 Sobek, J. and Schlapbach, R. (2004) Substrate architecture and functionality 9. define the properties and performance of DNA, peptide, protein, and carbohydrate microarrays. Pharmagenomics Sep. 15, 32–44. , and Sigrist, H. (2002) Protein density gradients on surfaces. 10. Langmuir 18, 2463–2467. 11 Steen, H. and Nørregaard Jensen, O. (2002) Analysis of protein-nucleic acid 11.

Recognit. 16, 165–176. 70 Gong, P. and Grainger, D. W. (2004) Comparison of DNA immobilization effi70. ciency on new and regenerated commercial amine-reactive polymer microarray surfaces Surface Sci. 570, 67–77. 71 Sobek, J. to be published. We thank Dr. J. Brunner, Environment and Health 71. Protection of the City of Zurich, for providing these data. 02_Sobek 5/5/07 10:43 AM Page 32 03_Sobek 5/5/07 10:44 AM Page 33 3 Optimization Workflow for the Processing of High Quality Glass-Based Microarrays Applications in DNA, Peptide, Antibody, and Carbohydrate Microarraying Jens Sobek, Catharine Aquino, and Ralph Schlapbach Summary As the performance of microarray experiments is directly dependent on the quality of the materials, the suitability of the protocols, and the accuracy of the work performed, optimization of existing microarray workflows is needed in almost every experiment to achieve higher quality and meaningfulness of the generated data.

Nature 416, 653–657. , et al. (2003). Staudinger ligation: a new immo56. bilization strategy for the preparation of small-molecule arrays. Angew. Chem. Int. Ed. 42, 5830–5834. , Park, S. , Koehler, A. , and Schreiber, S. L. (2003) 57. Expanding the functional group compatibility of small-molecule microarrays: discovery of novel calmodulin ligands Angew. Chem. Int. Ed. 42, 2376–2379. , et al. (2003) Immobilization of natural 58. products on glass slides by using a photoaffinity reaction and the detection of protein–small-molecule interactions.

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