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This 12-hour unfastened direction defined the functionality of the cytoskeleton and its position within the shipping of vesicles among subcellular cubicles.
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Young mouse sciatic nerve fiber showing developing myelin sheath. Note the relationships of the unit membranes of the mesaxon to the compact myelin. Magnification: X 160,000. 26 J . DAVID ROBERTSON that this must correspond to the radial repeating unit detected in myelin by X-ray diffraction studies. To be sure, the dimensions were reduced because of shrinkage during the preparatory procedures. The exact degree of shrinkage involved was studied by Finean  and later by Fernandez-Moran and Finean  by a combined X-ray diffraction and electron microscopic study that was of value in supporting the belief that the reduction in the radial repeat from about 170 Â to 180 Â to about 100 Â to 120 Â was due to shrinkage during preparation.
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From the table it can be seen that there is a reasonable correspondence between the total Cl~ uptake and the amount required to double the cytoplasmic mass and volume. Both Na and K are also taken up in adequate amounts, but because these ions might also be supplied by passive exchange across the cell membrane, we are less concerned TABLE I. Ion Uptake Compared to Ion Requirements during the Growth of Chaos chaos, Feeding on Paramecium aureliaa Na Ion content of fluid ingested Ion content of paramecia ingested Total uptake Requirement for growth a 3 14 77 1-2 K 15 95 110 90-100 Cl 30-45 14-18 44-63 60-67 Expressed in moles X 1 0 n .