Interleukin Protocols (Methods in Molecular Medicine) by Luke A. J. O'Neill (Editor), Andrew Bowie (Editor)

By Luke A. J. O'Neill (Editor), Andrew Bowie (Editor)

Luke O'Neill and Andrew Bowie collect a set of ordinary and complicated tools for measuring those strong brokers. Their easily reproducible innovations diversity from the assay of interleukin protein and mRNA-using ELISA, FACS, and RT-PCR-to the learn of interleukin sign transduction. more recent thoughts also are coated, together with the research of interleukin gene polymorphisms and using cDNA microarrays. a few of the assays are geared to precise pathologies, together with breast melanoma, melancholy, psoriasis, Grave's affliction, migraine, and myocardial infarction. entire and hugely useful, Interleukin Protocols deals biomedical investigators a stellar number of the entire significant recommendations had to examine the function of interleukins in ailment, to enhance prognosis, and to foster the speedy emergence of latest and extra strong therapeutics.

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By Luke A. J. O'Neill (Editor), Andrew Bowie (Editor)

Luke O'Neill and Andrew Bowie collect a set of ordinary and complicated tools for measuring those strong brokers. Their easily reproducible innovations diversity from the assay of interleukin protein and mRNA-using ELISA, FACS, and RT-PCR-to the learn of interleukin sign transduction. more recent thoughts also are coated, together with the research of interleukin gene polymorphisms and using cDNA microarrays. a few of the assays are geared to precise pathologies, together with breast melanoma, melancholy, psoriasis, Grave's affliction, migraine, and myocardial infarction. entire and hugely useful, Interleukin Protocols deals biomedical investigators a stellar number of the entire significant recommendations had to examine the function of interleukins in ailment, to enhance prognosis, and to foster the speedy emergence of latest and extra strong therapeutics.

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Coffman, R. , Beagley, K. , Eldridge, J. , et al. (1990) Detection of individual mouse splenic T cells producing IFN-a and IL-5 using the enzyme-linked immunospot (ELISPOT) assay. J. Immunol. Methods 128, 65–73. colored spots (indigo) are shown as the ideal profile of the spots corresponding to a Th0 cell (arrows) (original magnification ×40). , Development of a dual color enzyme-linked immunospot assay for simultaneous detection of murine T helper type-1- and T helper type 2-cells, pp. 107–116.

60: Interleukin Protocols Edited by: L. A. J. O'Neill and A. , Totowa, NJ 17 18 Hauer Fig. 1. Plates are coated with anti-cytokine and the captured cytokine is detected with enzyme-coupled antibody and visualized by addition of a chromogen. higher protein binding capacity, requirements for relatively large amounts of expensive coating reagents can be minimized, and nitrocellulose plates are now routinely used for cytokine analysis by the ELISPOT method (see Fig. 1). Furthermore, this method became a well-established technique for measuring cytokine synthesis, as it also has the benefit allowing cells secreting different cytokines to be compared with a common denominator, namely, the cell number.

1988) Enumeration of IFN-a producing human lymphocytes by spot-ELISA. A method to detect lymphokine-producing lymphocytes at the single cell level. J. Immunol. Methods 111, 25–34. 4. , Nilsson, L. , and Ouchterlony, Ö. (1988) Reverse ELISPOT assay for clonal analysis of cytokine production. 1. Enumeration of gamma-interferon-secreting cells. J. Immunol. Methods 110, 29–36. 5. Hauer, A. , Walker-Smith, J. , and MacDonald, T. T. (1997) The frequency of cells secreting interferon-a, interleukin-4, interleukin-5 and interleukin-10 in the blood and duodenal mucosa of children with cow’s milk hypersensitivity.

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