By Craig E. Lunte, William R. Heineman (auth.), E. Steckhan (eds.)
Contents/Information: C.E. Lunte, W.R. Heineman, Cincinnati, OH, united states: Electrochemical thoughts in Bioanalysis
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The 1st NATO technological know-how discussion board used to be held in Biarritz in September 1990. This Taormina convention is the second one in a sequence that we want to be an extended one and that i think that it has equalled the luck of its predecessor. In constructing those conferences the NATO technology Committee desired to assemble major specialists to study fields of sturdy current curiosity.
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Moody, G. , Thomas, J. D. R. : Selective Ion Sensitive Electrodes, Watford England: Merrow Publishing Co. Ltd. 1971 7. Koryta, J. : Ion-Selective Electrodes, Cambridge: Cambridge University Press 1975 8. Bailey, P. L. , London: Heyden 1980 9. Morf, W. E. : The Principles of Ion-Selective Electrodes and of Membrane Transport, New York: Am. Elsevier 1981 10. , Meier, P. C. : Ion-Selective Electrodes in Biology and Medicine, in: Frontiers of Chemistry (Laidler, K. ) New York: Pergamon Press, 1982, p.
The number of papers describing methods for catecholamine determination for certain circumstances are far too numerous to cite, however, several good reviews are available 196-198) The second major use of LCEC is in the determination of the hydroxyindote metabolites of tryptophan. Again several LCEC methods for determination of tryptophan metabolites have been reported 199-201) A recent paper by Seegal et al. 202)describes a general method to determine biogenic amines and metabolites in brain tissue, cerebral spinal fluid, urine and plasma.
Enzyme-linked immunoassays with electrochemical detection have been reported to provide even greater specificity and sensitivity than other enzyme linked electrochemical techniques. Enzyme linked electrochemical techniques can be carried out in two basic manners. In the first approach the enzyme is immobilized at the electrode. A second approach is to use a hydrodynamic technique, such as flow injection analysis (FIAEC) or liquid chromatography (LCEC), with the enzyme reaction being either off-line or on-line in a reactor prior to the amperometric detector.