By Ingrid C. Gelissen, Andrew J. Brown
This quantity presents state of the art suggestions for learning a variety of features of ldl cholesterol homeostasis, together with its uptake, synthesis and efflux from the cellphone, in addition to its trafficking in the telephone. Chapters additionally disguise innovations for learning the law of ldl cholesterol homeostasis at either the transcriptional and post-translational degrees, in addition to learning the membrane topology and constitution of cholesterol-related proteins. Written within the hugely profitable Methods in Molecular Biology series layout, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, comfortably reproducible laboratory protocols, and tips about troubleshooting and fending off identified pitfalls.
Authoritative and functional, Cholesterol Homeostasis: equipment and Protocols aims to supply key ideas in tackling the research of ldl cholesterol homeostasis.
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Extra info for Cholesterol Homeostasis: Methods and Protocols
Li H, Papadopoulos V (1998) Peripheraltype benzodiazepine receptor function in cholesterol transport. Identification of a putative cholesterol recognition/interaction amino acid sequence and consensus pattern. Endocrinology 139:4991–4997 43. Epand RM (2006) Cholesterol and the interaction of proteins with membrane domains. Jafurulla M, Tiwari S, Chattopadhyay A (2011) Identification of cholesterol recognition amino acid consensus (CRAC) motif in G-protein coupled receptors. Biochem Biophys Res Commun 404:569–573 45.
Taken together, these approaches have helped us address the molecular details of regulation of membrane protein function by cholesterol. Insights from such studies could help us understand details of functioning of important membrane proteins in healthy and diseased conditions with impaired cholesterol metabolism. ’s laboratory was supported by the Council of Scientific and Industrial Research (Govt. of India) Network project BSC0115. C. is an Adjunct Professor of Tata Institute of Fundamental Research (Mumbai), RMIT University (Melbourne, Australia), Indian Institute of Technology (Kanpur), and Indian Institute of Science Education and Research (Mohali).
Thus, a small amount of mevalonate (the product of HMGCR) is added to allow for the production of these products . 1) to decrease basal cholesterol levels. Cellular cholesterol can also be depleted using cyclodextrins, including methyl-β-cyclodextrin (MBCD) and (2-hydroxypropyl)-β-cyclodextrin (HPCD) . 2) or LDL, and this can be performed in media containing full serum, further enhancing cholesterol levels. , by statin pretreatment) to lower basal levels before treating with Chol/CD or LDL to maximize effects.